Forkhead box protein O1 (FoxO1) regulates lipids metabolism and cell proliferation mediated by insulin and PI3K-Akt-mTOR pathway in goose primary hepatocytes

In order to explore the role of forkhead box protein O1 (FoxO1) in lipid metabolism and cell proliferation, goose primary hepatocytes were isolated incubated with insulin or PI3K-Akt-mTOR pathway dual inhibitor NVP-BEZ235, then transfected FoxO1 interference plasmid. The related parameters proliferation measured. results firstly showed that increased intracellular TG lipids concentration (P < 0.05); proliferative index (PI), DNA synthesis, expression Cyclin D1 0.05). Secondly, co-treatment mRNA level content however, there was no significant difference between treatment miR-FoxO1 PI > Lastly, decrease induced by NVP-BEZ235 up-regulated significantly summary, could regulate mediated signaling hepatocytes. Further investigations are required highlight potential hepatocyte.